Hb estimation by sahli’s method ppt | Sahli’s method test procedure PPT.

Hb estimation by sahli’s method ppt | Sahli’s method Test procedure PPT. 


Hb estimation by sahli's method ppt. Hemoglobin Test procedure, sahli's method.
Hb estimation by sahli’s method ppt.

Hello friends if you search PPT of hemoglobin estimation by sahli’s method or HB estimation by sahli’s method PPT” then this is for you in a simple language and simple PPT of HB hemoglobin estimation by sahli’s method.


In this PPT we know about hemoglobin estimation by sahli’s method how it is performed and what is the normal value of hemoglobin how many types of hemoglobin estimation and many more.

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    What is hemoglobin?

    Hemoglobin is a protein molecules which is made of Heam(iron) and globulin(protein) which carry oxygen from the lungs.


    It’s made of protein molecules that is globupon  chain, the normal adult hemoglobin molecules, contain Two Alpha globulin and two beta globulin chain.

     Featus and infants have two Alpha globulin Chain and adult stage have Gamma globulin chain (beta Chain place to Gamma globulin chain).

    Hb estimation by sahli's method ppt. Hemoglobin Test procedure, sahli's method.
    HB estimation method PPT. 

    Estimation of haemoglobin in the laboratory –

    There are various methods are available for estimation of haemoglobin in the laboratory-

    Method based on the development of colour these are-

    • Sahli’s method or Acid hematin method
    • Cyanmethemoglobin method or colirimetric method
    • Oxyhemoglobin method
    • Alkaline method
    • Specific gravity method 
    • Gasometric method 
    Here we are know about sahli’s method estimation only ..


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    Hb estimation by sahli's method ppt. Hemoglobin Test procedure, sahli's method.
    HB estimation by sahli’s method PPT. 


    Hb estimation by sahli’s method ppt. Hemoglobin Test procedure, sahli’s method.

    Sahli’s method or acid-hematin method

    Principle of sahli’s method or acid heamatin method.

    Principle of sahli’s method :-Blood is mixed with N/10 HCL resulting in the conversion of HB to acid hematin which is brown in colour.The solution is diluted till its colour matches with the brown coloured glass of the comparator box the concentration of Hb is read directly.

    Requirement


    Haemocytometer which consists of

    • Comparator box which has brown coloured Glass on either side.
    • Hb pipette which is Mark upto 20 mm(0.02ml).
    • Tube with marking of HB on one side
    • Glass Road
    • Dropper
    • N/10 HCL
    • Distilled water and blood collection


    Procedure of hemoglobin estimation by sahli’s method-

    • First we take a clean and dry HB tube and egg and biotin HCL at the lowest level of HB tube.
    • After that we Tak 20 microlitre of whole blood in the tube and mix well.
    • Wait for 5 to 10 minutes or Allow to stand 5-10 minutes.
    • After that we match the colour with adding distilled water(drop by drop) in the tube(Hb tube) by glass road.
    • The result directly by noting the height of the diluted acid in and Express in g%.

    Note – The low(decrease) hemoglobin level shows as anaemia.

    Note – the high(increase) hemoglobin level shows as polycythemia.


    Hb estimation by sahli's method ppt. Hemoglobin Test procedure, sahli's method.
    Hb estimation by Sahli’s method PPT. 

    The advantages of sahli’s method-

    • Easy to perform
    • Quick result
    • Without electricity we perform this test
    • Can be used besides the procedure
    • Does not require a technical expert.

    Disadvantages of sahli’s method-

    • Less accurate
    • All hemoglobin are not converted to acid hematin and hence the value of HB obtained is less than the actual value.
    • The colours of acid hematin develops slowly slowly.
    • Colour of acid hematin fades with time and dilution must be done exactly after 10 minut when the colour development is maximum.
    • Individually variation in matching of color is seen.
    • Lack of true standard.
        

    Hb estimation by sahli's method ppt. Hemoglobin Test procedure, sahli's method.
    Normal range of hemoglobin PPT. 


    Normal range of hemoglobin-

    • In Male -14 to 18 gm% or gm/dl
    • In female -12 to 16gm% or gm/dl
    • In Infants -17 to 21gm% or gm/dl
    • In children -11 to 13 gm% or gm/dl

    Decrease hemoglobin level is referred as anaemia or low RBCs count.
    Increase hemoglobin level shows as polycythemia.

    Polycythemia – High production of Red blood cells (RBCs).

    Some commmon condition of low hemoglobin level-

    • Lots of blood traumatic injury, bleeding disorder ,surgery ,colon cancer ,stomach ulcer.
    • Nutritional deficiency (iron, folic acid (vitamin B12)).
    • Bone marrow problem (replacement of bone marrow).
    • Kidney failure
    • Abnormal structure of RBCs(sickle cell anemia, thelasemia).


    High hemoglobin level can be seen in

    • High altitude
    • smoke
    • Dehydration
    • polycythemia Vera( bone marrow produce high RBC or high production of red blood cells).

    Why N/10 HCL acid used in sahli’s method?

    If concentrated acid used in sahli’s method for Hemoglobin test, it gives always the same color of any patients hb, so for different Hemoglobin concentration it can be used for Hemoglobin test.



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